Captain America has no superhuman powers, but through the Super-Soldier Serum and "Vita-Ray" treatment, he is transformed and his strength, endurance, agility, speed, reflexes, durability, and healing are at the zenith of natural human potential. Rogers' body regularly replenishes the super-soldier serum; it does not wear off. The formula enhances all of his metabolic functions and prevents the build-up of fatigue poisons in his muscles, giving him endurance far in excess of an ordinary human being. This accounts for many of his extraordinary feats, including bench pressing 1200 pounds (545 kg) and running a mile (1.6 km) in 73 seconds (49 mph/78 kph). Furthermore, his enhancements are the reason why he was able to survive being frozen in suspended animation for decades. He is highly resistant to hypnosis or gases that could limit his focus. Rogers' battle experience and training make him an expert tactician and an excellent field commander, with his teammates frequently deferring to his orders in battle. Thor has stated that Rogers is one of the very few humans he will take orders from and follow "through the gates of Hades". Rogers' reflexes and senses are extraordinarily keen. He has blended judo, western boxing,kickboxing, and gymnastics into his own unique fighting style and is a master of multiple martial arts.

Iron Man possesses powered armor that gives him superhuman strength and durability, flight, and an array of weapons. The armor is invented and worn by Stark.

Thor is physically the strongest of the Asgardians. Thor possesses a very high resistance to physical injury that approaches invulnerability. His stamina allowed him to battle the entire Frost Giant army for nine months without any sustenance or rest. Thor has shown the ability to regenerate wounded portions of his body, including entire limbs or organs, with the aid of magical forces such as Mjolnir. Thor has superhuman speed, agility, and reflexes. Like all Asgardians, he has immunity to all Earthly diseases and some resistance to magic. Exceptionally powerful magic can overwhelm Odin's enchantment that transforms him between Asgardian and mortal forms.

Wolverine is a mutant with a number of both natural and artificial improvements to his physiology. His primary mutant power is an accelerated healing process, typically referred to as his mutant healing factor, that regenerates damaged or destroyed tissues of his body far beyond the capabilities of an ordinary human. In addition to accelerated healing of physical traumas, Wolverine's healing factor makes him extraordinarily resistant to diseases, drugs, and toxins. His healing factor also dramatically affects his aging process, allowing him to live far beyond the normal lifespan of a human. Despite being born in the late 19th century, he has the appearance, conditioning, health, and vitality of a man in his physical prime. While seemingly ageless, it is unknown exactly how greatly his healing factor extends his life expectancy. Wolverine's senses of sight, smell, and hearing are all superhumanly acute. He can see with perfect clarity at greater distances than an ordinary human, even in near-total darkness. His hearing is enhanced in a similar manner, allowing him to both hear sounds ordinary humans cannot and also hear to greater distances. Wolverine is able to use his sense of smell to track targets by scent, even if the scent has been eroded somewhat over time by natural factors.Wolverine's mutation also consists of animal-like adaptations of his body, including pronounced, and sharp fang-like canines and three retractable claws housed within each forearm.

While Hawkeye has no superhuman powers, he is at the very peak of human conditioning; he is an exceptional fencer, acrobat and marksman, having been trained from childhood in the circus and by the criminals. Hawkeye has also been thoroughly trained by Captain America in tactics, martial arts, and hand-to-hand combat.

When Cain Marko found the Gem of the mystical entity Cyttorak, he was empowered with magical energies and transformed into an immortal avatar for the entity in question. As the Juggernaut, Marko possesses superhuman strength, being capable of shattering mountains, lifting and using buildings as weapons,and extreme durability. The Juggernaut is described as physically unstoppable once in motion, does not tire from physical activity, and is able to survive without food, water, or oxygen. The Juggernaut heals quickly, as when he was stabbed through the eyes by Shatterstar, the wounds were healed almost immediately

Spider-Man has the ability to cling to walls, superhuman strength, a sixth sense ("spider-sense") that alerts him to danger, perfect balance and equilibrium, as well as superhuman speed and agility.

Wasp possesses the power to alter her physical size, causing her body's mass to be shunted to or gained from an alternate dimension known as Kosmos. She is able to shrink to a minimum of several centimeters or grow to a maximum of several hundred feet. Smaller or larger sizes are possible but the exertion puts a strain on her body. Initially, these abilities stem from use of a Pym particle gas released from special capsules, and later biochemical augmentation by Henry Pym. Over time, however, her body absorbs enough particles to cause cellular mutation due to repeated exposure to Pym particles, allowing her to alter her size at will. At miniature size, her strength level increases as her body's mass is compacted. At giant size, her strength and endurance increase geometrically with her height, reaching superhuman levels. Despite the advantages of giant size, Janet usually prefers to remain the diminutive Wasp, calling on her growth power only in times of extreme emergency. At miniature size, the Wasp grows a pair of translucent insect wings from her back, a result of genetic modifications provided by Hank Pym. These grant her the power of flight, at speeds up to 40 mph. The Wasp is able to harness and augment her body's natural bio-electric energy, releasing it from her hands in powerful electrical force bursts, which she calls her "stinger blasts", "stingers", or "wasp's stings". Originally, she requires special wrist devices to produce these, but again, Pym particle absorption allows her to create the ability unaided. The Wasp's genetic modifications also grant her the ability to sprout small prehensile antennae from her temples which allowed her to telepathically communicate with and control insects. However, this is an ability she rarely uses.

Luke Cage possesses superhuman strength and stamina, and has extremely dense skin and muscle tissue which render him highly resistant to physical injury. Cage possesses these abilities as a result of a cellular regeneration experiment which fortified the various tissues of his body. His skin can resist high caliber bullets, puncture wounds, corrosives, and extreme temperatures and pressures without sustaining damage. A second exposure to said experiments further enhanced his strength and durability. The same experiment which granted him his great strength and durability has also given him a faster than normal recovery time from injury. Luke Cage is an exceptional street fighter and was a gifted athlete before receiving superhuman abilities.

Sabretooth is a mutant with a number of natural mutant powers and artificial improvements to his physiology. His primary mutant power is an accelerated healing ability that allows him to regenerate damaged or destroyed areas of his body and cellular structure far beyond the capabilities of an ordinary human.Sabretooth possesses superhumanly acute senses that are comparable to those possessed by certain animals. Sabretooth is able to see objects with greater clarity and at much greater distances than an ordinary human. His sight is enhanced to the point that he can see with this same level of clarity in almost complete darkness, just like a nocturnal cat. This could be from being able to see into the infrared and ultraviolet portions of the spectrum. His sense of hearing is enhanced in a similar manner, allowing him to hear sounds that humans can not. Sabretooth is able to use his highly developed sense of smell to track targets by scent. He is able to track targets with an impressive degree of success, even if the scent has been eroded by natural factors such as weather conditions. His sense of taste is also developed to superhuman levels.Many of Sabretooth's natural physical attributes are beyond human levels. He possesses some degree of superhuman strength. The exact limits of his strength are unknown, but he could crush an iron barbell with ease. His mutant healing factor grants him superhuman stamina. He can push himself at peak capacity for several days before fatigue begins to affect him. His agility and reflexes were enhanced beyond the human body's natural limits. Combined with the ability to stalk, move quietly, and see in the dark he has the initiative in a fight.

Although the character is blind, his remaining four senses function with high levels of superhuman accuracy and sensitivity, giving him abilities far beyond the limits of a sighted person; few know that the hero cannot see. Daredevil developed a radar sense, which is similar to echolocation.

Plunging his fists into the molten heart of the dragon Shou-Lao the Undying infused the dragon's superhuman energy into Rand; this along with training by Lei Kung the Thunderer gave Rand the power of the Iron Fist, allowing him to summon and focus his chi (or natural energy) and enhance his natural abilities to extraordinary levels. His strength, speed, stamina, durability, agility, reflexes and senses can all be greatly intensified.